Hedgehog Signaling Pathway Database
 
RNAi Data
Jump to: Epistasis of ci with candidate Hh regulators Splicing Factors RNA Factors Nucleoporins Supplementary RNAi Data

 

Epistasis of ci with candidate Hh regulators

Locus tag Gene Hh score  Ci score Difference (Hh-Ci)
CG1708 cos -73 61 -134
CG1709 mts -62 41 -103
CG3412 slimb 36 127 -91
CG1135 CG1135 76 153 -77
CG18041 CG18041 45 121 -76
CG6292 CycT -77 -7 -70
CG6551 fu -73 -8 -65
CG1874 CG1874 -25 38 -63
CG9282 RpL24 -70 -8 -62
CG11561 smo -94 -34 -60
CG2184 Mlc2 -55 -4 -51
CG3193 Cm -72 -22 -50
CG17489 RpL5 -65 -16 -49
CG14813 COP -67 -17 -50
CG1475 RpL13A -83 -37 -46
CG6699 COP -73 -29 -44
CG1821 RpL31 -78 -35 -43
CG6625 Snap -52 -8 -44
CG10198 Nup98 -59 -16 -43
CG5179 Cdk9 -63 -20 -43
CG7961 aCOP -65 -23 -42
CG1528 gCOP -57 -16 -41
CG5931 CG5931 -64 -23 -41
CG7757 CG7757 -48 -8 -40
CG7923 Fad2 -48 -8 -40
CG32955 CG32955 -58 -19 -39
Insert brief discussion of work done in this screen, what it tells you and why it is important.

 

Splicing Factors
Top
Locus tag Gene Hh GL2 Hh GL3 Reverse PolIII-Hh Ci  No Hh  Potential second target(s) unction
CG3949 hoip -42.3 -75.6 -59.6 -71.4 19.5 113.4 0 RNA binding
CG7757 CG7757 -27.7 -47.7 -32.1 -36.8 -7.9 -30 0 U4/U6 snRNP
CG4602 Srp54 -35.4 -55 -25.5 -57.3 -26.7 -30.2 0 SR protein, splice site selection
CG2807 CG2807 -33.8 -67.9 -32.6 -51.1 -30.1 -33.1 0 U2 snRNP
CG8877 CG8877 -30.4 -71 -26 -35.4 -37.8 -34 0 U5 snRNP
CG3436 CG3436 -33.8 -65.6 -34.7 -70.4 -52.3 -35.8 0 U5 snRNP
CG3193 crn -41.5 -80.3 -50.9 -77.9 -46.7 -42.7 0 Splice site selection
CG3193 crn -44.4 -71.8 -56.1 -73.7 -21.9 -45.3 1 Splice site selection
CG6015 CG6015 -43.5 -76.4 -32.2 -71.5 -57 -54.1 0 WD repeat splicing factor
CG6987 SF2 -44.5 -69.2 178.1 -43 -82.9 -56 0 SR protein, splice site selection
CG7269 Hel25E -45.1 -57.5 -6.4 -42.5 -27.9 -4.8 0 Helicase, splicase site selection
CG3732 CG3732 -32.1 -56.6 0.2 -28.9 -45.8 -36.7 8 Zinc-finger splicing factor
CG5931 CG5931 -22.6 -64.1 -39.3 -42.3 -23.4 -19.5 0 U5 snRNP
CG16941 CG16941 -24.6 -60.1 -14.6 -42.6 -41.2 -34.9 0 U2 snRNP
CG3605 CG3605 -24.2 -67.3 -20.3 -36.1 -39.1 -36.9 0 U2 snRNP
CG6905 CG6905 -23 -57.6 -19.2 -39.8 -34.4 -21.3 0 Myb DNA-binding domain splicing factor
CG2925 noi -19.7 -67.9 -22.9 -44.1 -41.8 -38.1 0 U2 snRNP
CG18591 CG18591 -3.7 -47.2 -39.2 -21.8 -22.7 -14 0 Splicing factor

 

RNA Factors
Top

Locus tag Gene Hh GL2 Hh GL3 Reverse PolIII-Hh Ci  No Hh  Potential second target(s) Function
CG16901 sqd -35.2 -46 -28.8 -50.7 -15.4 -20.5 0 mRNA 3' binding
CG8435 CG8435 -36.7 -56.9 -38.8 -42.3 -34.9 -31.7 0 mRNA binding
CG2097 CG2097 -51.3 -63.3 -25.9 -54 -48.6 -48.2 0 mRNA cleavage, tight junction
CG5602 CG5602 -39.5 -50.4 -16.1 -51.1 -34 -20 0 DNA ligase/mRNA capping
CG7292 Rrp6 -34.7 -51.7 -3.5 -35.5 -60.7 -37.1 0 RNA exosome complex
CG6937 CG6937 -39.9 -59.5 -19.6 -36.9 -50 -37.9 1 RNA binding
CG4152 I(2)35Df -40.9 -57.1 -9.4 -46.4 -63.5 -47.4 0 RNA helicase
CG6694 CG6694 -9 -48.3 -22.3 -34 -45.8 5.6 0 RNA cleavage and polyadenylation
CG14641 CG14641 -19.8 -34 22.7 -3.9 -46.8 -29.1 0 RNA binding
CG4886 cyp33 15.7 -17.9 -46.6 -18.3 -17 2.8 0 RNA-binding protein
CG9748 bel 139.8 11 44.5 41.3 -3.5 17.1 3 RNA helicase
CG1101 Aly -14.8 -20.7 14.8 5.4 -48 -15.5 0 Exon junction complex

 

Nucleoporins
Top

Locus tag Gene Hh GL2 Hh GL3 Reverse PolIII-Hh Ci  No Hh  Potential second target(s) Function
CG10198 Nup98 -29.9 -59.1 -31.5 -36.7 -16.3 13.8 0 mRNA export
CG10198 Nup98 -48.8 -67.1 -50.4 -42.7 -28 2.3 0 mRNA export
CG4453 Nup153 -36.7 -47 -39.8 -36 -42.1 -0.2 0 mRNA export
CG4453 Nup153 -41.4 -62.1 -40.9 -43.8 -44 -10.2 0 mRNA export

 

 

 

A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway.

Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N.

Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA. knybakke@receptor.med.harvard.ed

Abstract: Members of the Hedgehog (Hh) family of signaling proteins are powerful regulators of developmental processes in many organisms and have been implicated in many human disease states. Here we report the results of a genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. The screen identified hundreds of potential new regulators of Hh signaling, including many large protein complexes with pleiotropic effects, such as the coat protein complex I (COPI) complex, the ribosome and the proteasome. We identified the multimeric protein phosphatase 2A (PP2A) and two new kinases, the D. melanogaster orthologs of the vertebrate PITSLRE and cyclin-dependent kinase-9 (CDK9) kinases, as Hh regulators. We also identified a large group of constitutive and alternative splicing factors, two nucleoporins involved in mRNA export and several RNA-regulatory proteins as potent regulators of Hh signal transduction, indicating that splicing regulation and mRNA transport have a previously unrecognized role in Hh signaling. Finally, we showed that several of these genes have conserved roles in mammalian Hh signaling.

Nat Genet. 2005 Dec;37(12):1323-32.

 
Last updated 28-Aug-2006

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